Comparison of PCR and a Swine Bioassay to Detect Hepatitis E Virus in Pig Tissues and Feces

نویسندگان

  • C. Kasorndorkbua
  • P. G. Halbur
چکیده

Swine hepatitis E virus (HEV) was detected by a semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in liver tissue and feces but not in skeletal muscle, pancreas, or heart from pigs experimentally infected intravenously with swine hepatitis E virus (swine HEV). Homogenates of liver tissue and suspensions of feces prepared from swine HEV-infected pigs were inoculated intravenously into naïve pigs and induced infection. There was no evidence of transmission of swine HEV to pigs by intravenous route of inoculation with heart or pancreas, or oral route with skeletal muscle homogenates or fecal suspensions prepared from HEV-infected pigs. Results indicate that there is potential for transmission of swine HEV to naïve pigs, and potentially to humans, via pig liver or liver cell xenotransplantation. Failure to detect HEV by RT-PCR in muscle tissue and failure to transmit swine HEV via oral inoculation of muscle tissue suggests that the risk of transmission of HEV in pork meat products is minimal. The route of natural transmission of HEV is thought to be fecal-oral so the failure to transmit HEV via feces suggests that a very high infectious dose is necessary, or there are other routes of transmission. The semiquantitative RT-PCR assay correlates well with that of in vitro swine bioassay.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular detection of hepatitis E virus in feces and slurry from swine farms, Rio Grande do Sul, Southern Brazil

Hepatitis E virus (HEV) is highly disseminated among swine herds worldwide. HEV is also a threat to public health, since particularly genotypes 3 and 4 may cause acute hepatitis in human beings. No previous studies were done on the occurrence of HEV in environmental samples in Rio Grande do Sul, Brazil. In the present study, reverse transcriptase-polymerase chain reaction (RT-PCR) was employed ...

متن کامل

Molecular detection of hepatitis delta virus in blood donors with RT-PCR

Abstract Background and Objective: Hepatitis delta virus is an imperfect virus with RNA and its activity depends on the presence of hepatitis B virus. This virus can lead to acute and chronic diseases in the liver. This study aimed to detect the hepatitis delta virus in blood donors with positive Hepatitis B Surface Antigens (HBsAg). Material and Methods: In this Study, 350 serum sa...

متن کامل

Detection of avian influenza virus of H9 subtype in the feces of experimentally infected chickens by RT–PCR

  Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...

متن کامل

Distribution and Propagation of Hepatitis E Virus in Experimentally Infected Swine

HEV infections in human and pigs have been reported in many countries; however, the precise distribution and multiplication of this virus in the host remains poorly understood. In this study, we examined the distribution and multiplication of HEV genotype 3 in two piglets at the early phase of intravenous infection, and also examined the virus distribution in a naturally infected pig. We develo...

متن کامل

Localisation of swine hepatitis E virus in experimentally infected pigs.

The distribution of intravenously inoculated swine hepatitis E virus (HEV) was assessed by in situ hybridisation for a period of 50 days. Evidence of apparent clinical disease was found in only one pig in the HEV infected group. The only gross lesion observed was mildly enlarged mesenteric lymph nodes at 50 days post infection (dpi). Histopathologically, mild lymphoplasmacytic infiltration and ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2017